Virus Imprinting.

The images below are Atomic Force Microscopy (AFM) topographies generated from the AMIPs™ research. As can be seen in Figures 1-4 (different samples), each of the stipples or bright spots is a single SARS-CoV-2 virus immobilized on the polymer-coated glass slide. This is used to create the final AMIPs™ detecting polymer. As can be seen in the figures the distribution of the viruses is relatively uniform and the number of viruses immobilized per square micron corresponds to our expected target density of imprints needed to achieve detection of the virus at clinically diagnostic levels.

Here is a video on how AFM works

Figure 1 – Virus Template

Figure 2 – Virus Template

Figure 3 – Virus Template

Figure 4 – Virus Template

Figure 5, is the actual Version 1 AMIPs™ polymer prototype. In this case, the the light blue patches are the virus captured AMIPs™ polymer prototype. As can be seen in comparison to the virus depositions and distribution represented in Figures 1-4, the virus was successfully transferred to the AMIPs™ polymer prototype. Again the density of viruses (number per square micron) transferred to the AMIPs™ polymer prototype corresponds closely to that of the immobilized polymer-coated glass slides indicating that the imprint stamp process was successful.

Figure 5 – Imprint Occupied by Viruses (Post Imprinting, Pre Elution)

The final step in making the AMIPs™ polymer prototype is removal or elution of the viruses from the polymer leaving it in a state ready to recapture the virus when exposed to it. Figures 6 and 7 are AFM topographies at different magnifications of the now revealed molecular imprints of the virus on the AMIPs™ polymer prototype. These and other samples are now ready to be tested for recapture and detection of the SARS-CoV-2 virus.

Figure 6 – Imprinting – Empty Cavities (Post Elution)

Figure 7 – Imprinting – Empty Cavities (Post Elution)

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